pchk2 thr68 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
pchk2 thr68
Pchk2 Thr68, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pchk2+thr68/Phospho-Chk2+(Thr68)+Rabbit+mAb/pm41535339-280-64-66
Average 96 stars, based on 545 article reviews
Pchk2 Thr68, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pchk2+thr68/Phospho-Chk2+(Thr68)+Rabbit+mAb/pm41535339-280-64-66
Average 96 stars, based on 545 article reviews
pchk2 thr68 - by Bioz Stars,
2026-10
96/100 stars
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Cell-Signaling:Article Title: CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor tolerance Article Snippet: pCHK1 (Ser345) , Cell Signaling , (Cell Signaling Technology Cat# 2348, RRID: AB_331212 ). .. Incubation:Article Title: Imidazolonylquinoline compounds and therapeutic uses thereof Article Snippet: .. After incubation for four hours at 37° C. and 10% of CO2, the cells are fixed (5 min, 4% formaldehyde in PBS), permeabilised (10 min, 0.2% Triton X-100 in PBS) and, after blocking of nonspecific binding sites (10% goat serum, 1% BSA in PBS), incubated overnight at 4° C. with a specific anti-pCHK2 antibody (cell signalling #2661). Article Title: ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance. Article Snippet: The protein was extracted by 12000 g centrifugation for 15 min at 4 C. The concentration of protein was detected by Pierce BCA Protein Assay Kit (#23225, Thermo) and recorded by Infinite M Nano System (Tecan) at 570 nm. .. Protein samples were loaded to 8%–12% SDS-PAGE gels, and run the samples with 80–120 V for 1 h. Then transferred into 0.22 μm PVDF membrane with 250 mA for 1.5–4 h. Blocked the membrane with 5% BSA in TBST for 1 h on a shaker and incubated with a specific primary antibodies pATR (S428) (1:1000, #178407, Abcam), ATR (1:1000, #13934S, CST), pChk1 (S345) (1:1000, #2348S, CST), Chk1 (1:1000, #32531, Abcam), pATM (S1981) (1:1000, #5883S, CST), ATM (1:1000, #2873S, CST), Article Title: Exploring the role of PARP1 inhibition in enhancing antibody–drug conjugate therapy for acute leukemias: insights from DNA damage response pathway interactions Article Snippet: The percentage of phospho-H2AX-positive cells was quantified using Attune Nxt Flow cytometer (ThermoFisher Scientific) The remaining cells were cultured for additional 21 h (total 24 h) and then counted by Trypan blue exclusion assay (Sigma Aldrich) to evaluate the cell viability. .. Immunoblotting analyses were performed using Mini-Protean TGX stain-free precast gels, blotted to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) and incubated the following antibodies: pATR Ser428 (#2853); pATM Ser1981 (#5883); Blocking Assay:Article Title: Imidazolonylquinoline compounds and therapeutic uses thereof Article Snippet: .. After incubation for four hours at 37° C. and 10% of CO2, the cells are fixed (5 min, 4% formaldehyde in PBS), permeabilised (10 min, 0.2% Triton X-100 in PBS) and, after blocking of nonspecific binding sites (10% goat serum, 1% BSA in PBS), incubated overnight at 4° C. with a specific anti-pCHK2 antibody (cell signalling #2661). Binding Assay:Article Title: Imidazolonylquinoline compounds and therapeutic uses thereof Article Snippet: .. After incubation for four hours at 37° C. and 10% of CO2, the cells are fixed (5 min, 4% formaldehyde in PBS), permeabilised (10 min, 0.2% Triton X-100 in PBS) and, after blocking of nonspecific binding sites (10% goat serum, 1% BSA in PBS), incubated overnight at 4° C. with a specific anti-pCHK2 antibody (cell signalling #2661). other:Article Title: APOBEC3B enhances the efficacy of PARP inhibitors in elimination of ovarian cancer stem cell Article Snippet: The following antibodies were used: APOBEC3B (Abcam, Cat #ab184990; RRID:AB_2891094); CD133-APC (Biolegend, Cat #397,906; RRID:AB_2876721); SOX2 (Abcam, Cat #ab92494; RRID:AB_10585428); PARP (Abcam Cat #ab191217; RRID:AB_2861274); Cleaved PARP (Abcam Cat #ab32561; RRID:AB_777103); γH2AX (S139) (Abcam, Cat #ab11174; RRID:AB_297813); Chk1 (Abcam, Cat #ab40866; RRID:AB_726820); pChk1 (S317) (Abcam, Cat #ab278717); UNG (Origene, Cat #TA503563; RRID:AB_11126624); SDS Page:Article Title: ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance. Article Snippet: The protein was extracted by 12000 g centrifugation for 15 min at 4 C. The concentration of protein was detected by Pierce BCA Protein Assay Kit (#23225, Thermo) and recorded by Infinite M Nano System (Tecan) at 570 nm. .. Protein samples were loaded to 8%–12% SDS-PAGE gels, and run the samples with 80–120 V for 1 h. Then transferred into 0.22 μm PVDF membrane with 250 mA for 1.5–4 h. Blocked the membrane with 5% BSA in TBST for 1 h on a shaker and incubated with a specific primary antibodies pATR (S428) (1:1000, #178407, Abcam), ATR (1:1000, #13934S, CST), pChk1 (S345) (1:1000, #2348S, CST), Chk1 (1:1000, #32531, Abcam), pATM (S1981) (1:1000, #5883S, CST), ATM (1:1000, #2873S, CST), Membrane:Article Title: ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance. Article Snippet: The protein was extracted by 12000 g centrifugation for 15 min at 4 C. The concentration of protein was detected by Pierce BCA Protein Assay Kit (#23225, Thermo) and recorded by Infinite M Nano System (Tecan) at 570 nm. .. Protein samples were loaded to 8%–12% SDS-PAGE gels, and run the samples with 80–120 V for 1 h. Then transferred into 0.22 μm PVDF membrane with 250 mA for 1.5–4 h. Blocked the membrane with 5% BSA in TBST for 1 h on a shaker and incubated with a specific primary antibodies pATR (S428) (1:1000, #178407, Abcam), ATR (1:1000, #13934S, CST), pChk1 (S345) (1:1000, #2348S, CST), Chk1 (1:1000, #32531, Abcam), pATM (S1981) (1:1000, #5883S, CST), ATM (1:1000, #2873S, CST), Western Blot:Article Title: ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance. Article Snippet: The protein was extracted by 12000 g centrifugation for 15 min at 4 C. The concentration of protein was detected by Pierce BCA Protein Assay Kit (#23225, Thermo) and recorded by Infinite M Nano System (Tecan) at 570 nm. .. Protein samples were loaded to 8%–12% SDS-PAGE gels, and run the samples with 80–120 V for 1 h. Then transferred into 0.22 μm PVDF membrane with 250 mA for 1.5–4 h. Blocked the membrane with 5% BSA in TBST for 1 h on a shaker and incubated with a specific primary antibodies pATR (S428) (1:1000, #178407, Abcam), ATR (1:1000, #13934S, CST), pChk1 (S345) (1:1000, #2348S, CST), Chk1 (1:1000, #32531, Abcam), pATM (S1981) (1:1000, #5883S, CST), ATM (1:1000, #2873S, CST), Article Title: Exploring the role of PARP1 inhibition in enhancing antibody–drug conjugate therapy for acute leukemias: insights from DNA damage response pathway interactions Article Snippet: The percentage of phospho-H2AX-positive cells was quantified using Attune Nxt Flow cytometer (ThermoFisher Scientific) The remaining cells were cultured for additional 21 h (total 24 h) and then counted by Trypan blue exclusion assay (Sigma Aldrich) to evaluate the cell viability. .. Immunoblotting analyses were performed using Mini-Protean TGX stain-free precast gels, blotted to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) and incubated the following antibodies: pATR Ser428 (#2853); pATM Ser1981 (#5883); Article Title: Repurposing the anthelmintic drug mebendazole in combination with radiation therapy in an isocitrate dehydrogenase mutant glioma model Article Snippet: Protein concentration was measured using the BCA protein kit assay (ThermoFisher). .. Western blot was performed as previously described., The following primary antibodies were used: pAurora B(thr232), Aurora, pATM(ser1981), ATM, BAK, BAX, BCL2, pCDK1(tyr15), pChk2( Imaging:Article Title: ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance. Article Snippet: The protein was extracted by 12000 g centrifugation for 15 min at 4 C. The concentration of protein was detected by Pierce BCA Protein Assay Kit (#23225, Thermo) and recorded by Infinite M Nano System (Tecan) at 570 nm. .. Protein samples were loaded to 8%–12% SDS-PAGE gels, and run the samples with 80–120 V for 1 h. Then transferred into 0.22 μm PVDF membrane with 250 mA for 1.5–4 h. Blocked the membrane with 5% BSA in TBST for 1 h on a shaker and incubated with a specific primary antibodies pATR (S428) (1:1000, #178407, Abcam), ATR (1:1000, #13934S, CST), pChk1 (S345) (1:1000, #2348S, CST), Chk1 (1:1000, #32531, Abcam), pATM (S1981) (1:1000, #5883S, CST), ATM (1:1000, #2873S, CST), Staining:Article Title: Exploring the role of PARP1 inhibition in enhancing antibody–drug conjugate therapy for acute leukemias: insights from DNA damage response pathway interactions Article Snippet: The percentage of phospho-H2AX-positive cells was quantified using Attune Nxt Flow cytometer (ThermoFisher Scientific) The remaining cells were cultured for additional 21 h (total 24 h) and then counted by Trypan blue exclusion assay (Sigma Aldrich) to evaluate the cell viability. .. Immunoblotting analyses were performed using Mini-Protean TGX stain-free precast gels, blotted to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) and incubated the following antibodies: pATR Ser428 (#2853); pATM Ser1981 (#5883); |